BACKGROUND INFORMATION AND APPLICATIONS
CHO TF is a complete chemically-defined, animal-component-free medium. CHO TF was developed by Xell for cultivation of CHO and other mammalian cell lines, with a special focus on transfection applications and virus production. The medium is especially suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI). CHO TF supports cell growth and production of recombinant proteins and antibodies in suspension culture. It can be used in research or in manufacturing application.?
??Chemically defined, serum-free, animal component-free and hydrolysate-free
??Little or no adaptation required from other serum-free media
??Supports stable growth of suspension cells at high viability in seed train culture
??Compatibility with cationic transfection reagents (e.g. PEI) facilitates simplified transfection
??Liquid for R&D is manufactured at Xell
Components and specifications
??CHO TF medium
??Free of animal-derived components
??Free of proteins
??Free of growth hormones
??Store protected from light at 2?8 °C. Do not freeze.
??CHO TF is intended for research or further manufacturing purposes only. It is not intended for human or animal diagnostic or therapeutic use.
RELATED APPLICATION EXAMPLE
FIG.1: Cultivations of CHO cell line 2 (grey) and CHO cell line 3 (orange)?expressing a monoclonal antibod?y (mAb) in the final CHO TF medium?formulation. Titers were measured from day 4 post-transfection onwards?and reached 70 mg/L and 110 mg/L, respectively. For transfection, a?simple protocol without the use of feeds, temperature shift or typically?used enhancers was applied.